|
|
Preprocessing of 1D gel electrophoresis image
Jaroš, Luboš ; Škutková, Helena (referee) ; Vítek, Martin (advisor)
Gel electrophoresis is an important method used for separation and analysis of macromolecules with electric potential. This well-established method has a big potential in medicine and science, however, the output images often suffer from distortion. This work describes several known sources of distortion and elaborates on existing methods of the track detection in gel electrophoresis. The core of this thesis introduces a new method to detect individual tracks and image pre-processing based on the standard deviation calculated on the horizontal segmentation of the image. The proposed algorithm is implemented and tested in Matlab. The success rate of the proposed method reaches 96,7 % on 35 test images of gel electrophoresis. The next phase is the band detection in the vertical profile and the binary image drawing. Although the band detection is not fully automated, a detection success rate achieves up to 86,4 % when proper setting is used.
|
|
|
Mobile application for electrophoretic gel image processing
Mojžišová, Anna ; Čejková, Darina (referee) ; Škutková, Helena (advisor)
Analýza obrazů 1D gelové elektroforézy je klíčovým krokem ve výzkumu molekulární biologie a umožňuje identifikaci a kvantifikaci proteinů, fragmentů DNA a dalších molekul. Tradiční proces analýzy však může být časově náročný a vyžaduje specializovaný software a vybavení. Tato bakalářská práce představuje nové řešení tohoto problému zavedením mobilní aplikace, která pomáhá při analýze obrazů 1D gelové elektroforézy. Aplikace poskytuje uživatelsky přívětivé rozhraní a sadu výkonných nástrojů pro zpracování obrazu, , segmentaci drah, detekci proužků a aproximaci molekulové hmotnosti. Navrhované metody byly podrobně testovány na sadě různých gelových snímků a výsledky ukazují, že aplikace dokáže přesně a efektivně analyzovat elektroforetické snímky. Přenosnost aplikace umožňuje výzkumným pracovníkům provádět analýzy na cestách, čímž se snižuje čas a náklady spojené s tradičními metodami analýzy. Tato mobilní aplikace představuje pohodlný a praktický nástroj pro výzkumné pracovníky i lékaře, díky němuž je analýza elektroforetických obrazů dostupnější a efektivnější.
|
|
|
Preprocessing of 1D gel electrophoresis image
Jaroš, Luboš ; Škutková, Helena (referee) ; Vítek, Martin (advisor)
Gel electrophoresis is an important method used for separation and analysis of macromolecules with electric potential. This well-established method has a big potential in medicine and science, however, the output images often suffer from distortion. This work describes several known sources of distortion and elaborates on existing methods of the track detection in gel electrophoresis. The core of this thesis introduces a new method to detect individual tracks and image pre-processing based on the standard deviation calculated on the horizontal segmentation of the image. The proposed algorithm is implemented and tested in Matlab. The success rate of the proposed method reaches 96,7 % on 35 test images of gel electrophoresis. The next phase is the band detection in the vertical profile and the binary image drawing. Although the band detection is not fully automated, a detection success rate achieves up to 86,4 % when proper setting is used.
|
|
|
Changes of protein profile in barley during malting
Šopíková, Martina ; Mazanec,, Karel (referee) ; Bobáľová,, Janette (advisor)
This diploma thesis is focused on studies of changing of protein profile during barley malting. Substantial part of this work is devoted to the proteomics identification of barley proteins which change during malting and so become more stationary and they influence quality of beer (haze and foam in beer). For this experiment was used barley variety Jersey. In the theoretical part of this thesis there is information about beer, manufacturing of beer with description of important commodities for manufacturing of beer and information about barley malting and information about malting process. Next there is description of methods for separation of proteins (1D gel electrophoresis and 2D gel electrophoresis), MALDI TOF/TOF mass spectrometry and this use for the analysis and identification of proteins, the use of matrices and ways of the sample preparation. In the experimental part of this thesis there was carried out the optimisation of the dosage of sample for 1D gel electrophoresis and the optimisation of staining. The 15 % TRIS-HCl gel was the best, this gel was stained by Commassie Brilliant Blue G-250. For illustration of changes was made 2D gel electrophoresis. With help of method peptide mass fingerprinting and MS/MS protein of barley – protein Z, -amylase subtilisin inhibitor, -amylase a peroxidase were identificated. The analysis of barley extract intact proteins was carried out, this analysis was focused on changes of important barley protein LTP 1.
|
guest ::
